Tomography
Overview
Hummingbird Scientific’s single-tilt tomography TEM holder with removable sample clamp tips are designed to maximize tilt and allow cross-platform transfers and imaging. Because the system permits unusually high tilt angles, the sample itself is the only contributor to the missing wedge. A range of removable tips are available for the system and can be customized for specific specimen support geometries.
Sample Applications
- 3D reconstruction of nanostructures and embedded structures (e.g. dislocations)
- Correlative comparisons between TEM and atom probe tomography
- 3D reconstruction of embedded biological specimens
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Single Point TipA dedicated sample holder for needle samples. |
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1mm Grid TipClamps a 1mm TEM sample for high-tilt tomography applications in narrow, gap-objective lens microscopes |
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3mm Full Grid TipClamps a standard 3mm TEM sample with minimal shadowing at high alpha-tilt angles. |
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3mm Half Grid TipSpecifically designed for holding TEM half grids. The sample clamp itself does not shadow the sample. |
Atom Probe Tomography Workflow
One sample on one tip: using our TEM tomography holder system in combination with a Focus Ion Beam (FIB) and Atom Probe (AP), researchers have a comprehensive, cross-correlative view for 3D sample reconstruction at the atomic and nano scale. The sample is first sectioned out to the required size and shape using the FIB. The sample can then be left on the same tip and transferred to the TEM for final shape verification and tilt-series acquisition before being placed in the Atom Probe for final analysis.
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Accessories
Accessories available for your tomography holder include:
- 1mm Square Grids
- 1mm L-Grids
- 3mm Tabbed Grids
- Half-Grid Setup Block
- Full-Grid Setup Block
Contact us for more information.
EditRelated Products
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Liquid Holder See MoreThe liquid holder is a complete in-situ TEM lab system, designed to enable high-resolution material characterization in liquids. The system offers single-inlet, dual-inlet, heating, electrochemical, spectroscopy, and cross-correlative features. Perform post-mortem imaging in vacuum using the tomography holder and the special liquid-cell chip tip. |
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Cylindrical confinement used as a self assembly technique for nanoparticles
Direct assembly of nanosized building blocks into a functional entity has been a great challenge, especially for DNA templating, which requires aqueous solution. Here, researchers from the University of California, Berkeley and Lawrence Berkeley National Lab were able to use Hummingbird Scientific’s Tomography holder to observe 3D cylindrical confinement of nanoparticles and their self-assemblies into stacked rings and single and double helices.
Reference: Ting Xu et al. Diversifying Nanoparticle Assemblies in Supramolecule Nanocomposites Via Cylindrical Confinement. Nano Letters (2017). Abstract
Copyright © 2017 American Chemical Society
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3D analysis of Au nanoparticle helical assembly. Image copyright © 2017 American Chemical Society
Tilt series Z-contrast STEM images of a Ge nanowire after alignment into a rotation projection, taken using Hummingbird Scientific’s Tomography holder. The nano-wire is approximately 150nm in diameter and it shows positioning of a gold nano-particle on the wire.
Reference: J. Wu, S. Padalkar, S. Xie, E.R. Hemesath, J. Cheng, G. Liu, A. Yan, J.G. Connell, E. Nakazawa, X. Zhang, L.J. Lauhon, V.P. Dravid. “Electron Tomography of Au-Catalyzed Semiconductor Nanowires,” Journal of Physical Chemistry C 117:2 (2013) pp.1059‒1063. Abstract
Copyright © 2012, American Chemical Society
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