Hummingbird SEM Liquid Stage in Microscope

Liquid Flow

The World Leader in Environmental Cell Microscopy

Technical Specs

Hummingbird Scientific SEM Stage - ElectroChemistry Liquid Flow Cell - Cross Correllative TEM Holder
1400 Series SEM
Number of Inlets 1 or 2 depending on model, single outlet
Biasing Contacts 4
Tubing Type User-replaceable microfluidic tubing
Delivery System Variable-speed liquid delivery system
Tip Type Removable tip
Flow Type Continuous or static liquid flow
SEM Compatibility Custom integration*

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Features

Featured Research

Cross-Correlative Studies

Researchers studying the interaction of biological structures with the environment have long been hindered by the lack of a single microscopy technique capable of spanning all relevant length scales. In response to this problem, we have developed systems that allow multiple correlative imaging techniques. The SEM liquid holder enables researchers to perform cross-correlative experiments and to image specimens in liquid environment. Using this system, researchers at Evergreen State College, Ames Laboratory, and the University of Georgia have successfully taken correlative images of biological specimens using LM, SEM, and TEM.

Figure: Correlating fluorescence (top) and SEM images of autofluorescent Anthrospira in phosphate-buffered saline (bottom), both taken using Hummingbird’s correlative liquid cell system.

Reference: D.A. Fischer, D.H. Alsem, B. Simon, T. Prozorov and N. Salmon. “Development of an Integrated Platform for Cross-Correlative Imaging of Biological Specimens in Liquid using Light and Electron Microscopies.” Microscopy and Microanalysis 19:Suppl. 2 (2013) pp. 476–477. Abstract

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Selected Publications

D.A. Fischer, D.H. Alsem, B. Simon, T. Prozorov, and N. Salmon. “Development of an Integrated Platform for Cross-Correlative Imaging of Biological Specimens in Liquid using Light and Electron Microscopies.” Microscopy and Microanalysis 19:Suppl. 2 (2013), pp. 476–477 Abstract

 

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