|1400 Series SEM|
|Number of Inlets||1 or 2 depending on model, single outlet|
|Biasing Contacts||3 or 4 depending on model|
|Tubing Type||Replaceable microfluidic tubing|
|Delivery System||Variable-speed liquid delivery system|
|Tip Type||Removable tip|
|Flow Type||Continuous or static liquid flow|
|SEM Compatibility||Custom integration|
Hummingbird Scientific’s SEM liquid-cell system allows real-time imaging of solid-liquid interfaces in scanning electron microscopes. The system mount uses the same removable tip design as our liquid TEM holder, allowing for cross-correlative experiments across optical and electron microscope platforms. The two-chip fluid cell allows users to quickly and easily prepare and exchange samples while remaining confident in the cell’s seal.
- Imaging biological specimens in liquid environments
- Liquid-electrochemistry experiments
- In-situ hydration experiments
Left: Hummingbird Scientific’s SEM liquid holder for JEOL microscopes (left) and FEI microscopes (right, mounted on assembly stand).Read MoreEdit
How it Works
Hummingbird Scientific’s SEM liquid system encloses the sample within a microfabricated liquid cell in the holder tip, which is sealed from the microscope chamber environment by a patented sealing mechanism. The fluid pump and power supply are located safely outside of the chamber. A customizable chamber interface and vacuum sealed supply system provide fluid and power to the tip. Using Hummingbird’s two-chip fluid cell, users can prepare their sample of choice using the same methods they would for any other electron transparent membrane substrate. The tip can be swapped between light microscopes, the SEM, and the TEM for cross-correlative studies.Read MoreEdit
Our liquid system’s unique removable holder tip allows for correlative imaging of transmission electron, scanning electron, X-ray, and optical microscopy of samples in liquid environments. Dedicated holders are available for each technique. All holders can interface with the same liquid-cell tip for site-specific imaging.
Our SEM liquid holder is compatible with most TEM liquid-cell options. Common options include:
- Continuous Flow
- Dual Flow/Mixing
- Static Cell
- Vapor System
Don’t see what you’re looking for? We would also be happy to develop a custom solution for you.Edit
Researchers studying the interaction of biological structures with the environment have long been hindered by the lack of a single microscopy technique capable of spanning all relevant length scales. In response to this problem, we are working to develop systems that combine two or more imaging techniques. The SEM liquid holder, for example, enables researchers to perform cross-correlative experiments and to image specimens in the ambient environment. Using the system, researchers at Evergreen State College, Ames Laboratory, and the University of Georgia have successfully taken correlative images of biological specimens using LM, SEM, and TEM.
Figure: Correlating fluorescence (top) and SEM images of autofluorescent Anthrospira in phosphate-buffered saline (bottom), both taken using Hummingbird’s correlative system.
Reference: D.A. Fischer, D.H. Alsem, B. Simon, T. Prozorov and N. Salmon. “Development of an Integrated Platform for Cross-Correlative Imaging of Biological Specimens in Liquid using Light and Electron Microscopies.” Microscopy and Microanalysis 19:Suppl. 2 (2013) pp. 476–477. AbstractEdit