Electron holography of bacterial cells in liquid

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Researchers at U.S. Ames Laboratory, Imperial College  London and Ernst Ruska-Center for Microscopy and Spectroscopy with Electrons and Peter Grunberg Institute have used Hummingbird Scientific’s Liquid Cell platform to demonstrate for the first time the holography imaging of bacterial and magnetic particles in liquid. The findings are published in Journal of the Royal Society Interface.

off axis holography

Copyright © 2017 The Royal Society Publishing

The effect of electromagnetic fields in the biological system has been poorly understood.  The researcher used Hummingbird’s Liquid cell holder to perform off-axis holography imaging of hydrated cells of Magentospirillum magneticum strain AMB-1 and assemblies of magnetic nanoparticles. They were able to capture electron holograms to show interference fringe contrast to allow reconstruction of the phase shift of the electron wave and mapping of the magnetic induction from bacterial magnetite nanocrystals. The development of this technique could potentially help  in the future studies of solid-liquid interfaces, biomineralization and protein aggregation.

Reference: Tanya Prozorov, Trevor P. Almeida, András Kovács, and Rafal E. Dunin-Borkowski. ” Off-axis electron holography of bacterial cells and magnetic nanoparticles in liquid,” Journal of The Royal Society Interface (2017). DOI: 10.1098/rsif.2017.0464


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