Liquid System Cross-Correlative Imaging

Hummingbird TEM SEM Optical Correlative Liquid Cell

Our liquid system’s unique removable holder tip allows for correlative imaging of transmission electron, scanning electron, X-ray, and optical microscopy of samples in liquid environments.  Dedicated holders are available for each technique.  All holders can interface with the same liquid-cell tip for site-specific imaging.

Correlative imaging allows researchers to acquire data across different length scales.  It also allows for the acquisition of complementary data:  high-resolution internal microstructure with TEM and X-ray, surface topography with high magnification and high depth of field in SEM, and lower magnification screening with optical microscopy.  And it supports the use of different spectroscopy techniques, including fluorescence, X-ray absorption spectroscopy, energy-dispersive X-ray spectroscopy (EDS), and electron energy loss spectroscopy (EELS).

Cross Correlative Imaging Selected Publications

T.J. Woehl, S. Kashyap, E. Firlar, T. Perez-Gonzalez, D. Faivre, D. Trubitsyn, D.A. Bazylinski and T. Prozorov. “Correlative Electron and Fluorescence Microscopy of Magnetotactic Bacteria in Liquid: Toward In Vivo Imaging” Scientific Reports 4, 6854 (2014) Abstract
D.A. Fischer, D.H. Alsem, B. Simon, T. Prozorov and N. Salmon. “Development of an Integrated Platform for Cross-Correlative Imaging of Biological Specimens in Liquid using Light and Electron Microscopies.” Microscopy and Microanalysis, 19 (Suppl. 2) , pp 476-477. (2013) Abstract


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